Let me start with a question and suggestion…
Are you new to developing accessible e-Learning in Lectora? If so, learning how to do this from the ground-up would be beneficial for you.
Without really seeing what’s going on ‘under the hood’, any responses here should be considered only possibilities. For example, depending on how your Title is set up, the answer to your question could impact several things that you’re doing. Like, are heading styles being used (as LL mentioned). Other settings can impact read order too, like Always On Top and Anchor Position. You mentioned IE – does this happen in all Browsers and does the behavior change across screen readers? Have you cleared the cache, etc. Some research may be necessary.
It’s also important to understand that checking the Use Web Accessibility settings isn’t going to make your title accessible. It is only going to provide a high-level check of your content.
My suggestion is that you join the Lectora Accessibility User Group to participate in monthly discussions. When you join the LAUG http://thelaug.org/index.html, you will be added to my email distribution list and receive notices of our online monthly discussions. The next one is on March 26, 2020.
In addition to the LAUG monthly discussions, we also have a group here on the Trivantis Comminity that you can join. It may be helpful for you in-between meetings to post questions and answers for others. https://community.trivantis.com/groups/lectora-accessibility-user-group-laug/